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To address diseases brought on by bacteria that are resistant to antibiotics, bacteriophages—viruses that particularly target and destroy bacteria—are utilized as an alternative to antibiotics. In this study, phages from hospital sewage were isolated and characterized in relation to the clinical strain of multidrug-resistant Staphylococcus aureus. The phages were isolated and identified using biochemical methods, including coagulase tests, catalase tests, microscopy, and mannitol fermentation on mannitol salt agar. When antibiotic resistance was evaluated for these isolated bacteria, it was shown that all isolates had no resistance to Vancomycin, but they were 100% resistant to Augmentin, 68% to Ciprofloxacin, 53% to Cefotaxime, 50% to Cefixime, 33% to Streptomycin, 30% to Chloramphenicol, and 13% to Oxacillin. Consequently, specific phages identified as PA, PB, and PC were isolated and characterized for further study. The PA phage showed a broad host range in the host range analysis, lysing eight of the twenty different strains of Staphylococcus while not infecting any other bacteria. While a notable drop in titer was noted at pH levels 5 and 9, this phage maintained stability at temperatures as high as 60 °C and showed peak activity at pH 7. Investigating the biological properties of these phages can provide valuable insights and contribute to the development of potential therapeutic agents against multidrug-resistant Staphylococcus aureus.

Keywords: Bacteriophages, Antibiotic Resistance, MDR, Staphylococcus.