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The present work deals with the scale up of adsorption column for the removal of basic dyes from wastewaters, using methylene blue as a representative adsorbate and barley straw as a representative waste biomass adsorbent. The pilot scale column used herein was 137 cm in height with 19 cm inner diameter. The flow rate was determined so that the linear velocity for the pilot scale column is approximately the same with the linear velocity for the two laboratory scale columns (15 and 25 cm in height, respectively, with 2.5 cm inner diameter). In the case of the pilot scale column the time required for breakthrough (predetermined at C=2 mg/L) was 140 min and the corresponding required influent volume was 220 L (Ci=14 mg/L), where the C, Ci are concentrations for adsorbate output and input, respectively. The results indicate that barley straw exhibits satisfactory adsorption properties. Considering the abundance and the low cost of this lignocellulosic material, barley straw could serve as a cost-effective adsorbent for basic dyes. Key Words: Adsorption, Barley Straw, Packed Bed Column, Methylene Blue, Scale Up.